OCTN2 Transporter Assay (SLC22A5)

The OCTN2 transporter is a cation/zwitterion sodium dependent high affinity carnitine transporter. It is localized widely in many organs including the kidneys, pancreas, prostate, skeletal muscle and heart.

Genetic defects affecting OCTN2 are responsible for conditions including hypoglycemia and carnitine deficiency which can lead to rhabdomyolysis, cardiomyopathy and heart failure. OCTN2 Substrates

Optivia offers OCTN2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for OCTN2 inhibitors as well as Vmax and Km values for OCTN2 substrates.

OCTN2 Transporter Assay

Relevance – renal tubular reabsorption, critical genetic polymorphism Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Carnitine
Subcellular Localization in Assay Model – Apical Inhibition Positive Control – Verapamil