OCT2 Transporter Assay (SLC22A2)

The OCT2 transporter is an organic cation uptake transporter that is encoded by the SLC22A2 gene. OCT2 is expressed in the kidney on the basal-lateral membrane of proximal tubule epithelial cells. Because it serves to take substrates from the blood stream into the kidney, OCT2 can mediate the renal elimination of these compounds.

Optivia offers OCT2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for OCT2 inhibitors as well as Vmax and Km values for OCT2 substrates.

 OCT2 Transporter Assay

Relevance – FDA & EMA guidance; renal uptake Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human, rat Probe Substrate – Metformin
Subcellular Localization in Assay Model – Basolateral Inhibition Positive Control – Quinidine