OAT3 Transporter Assay (SLC22A8)

The OAT3 transporter is an organic anion uptake transporter that is encoded by the SLC22A8 gene. OAT3 is expressed in the kidney on the basal-lateral membrane of proximal tubule epithelial cells. Because it serves to take compounds into the kidney from the blood stream, OAT3 can mediate the renal elimination of these compounds.

Optivia offers OAT3 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for OAT3 inhibitors as well as Vmax and Km  for OAT3 substrates.

The validated Optivia OAT3 assay uses estrone-3-sulfate as the probe substrate and probenecid as the reference inhibitor.

OAT3 Transporter Assay

Relevance – FDA & EMA guidances; renal uptake Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Estrone-3-sulfate or p-Aminohippurate
Subcellular Localization in Assay Model – Basolateral Inhibition Positive Control – Probenecid