NTCP Transporter Assay (SLC10A1)

The NTCP transporter is encoded by the SLC10A1 gene. It is also known as the sodium (Na+) taurocholate co-transporting polypeptide. NTCP is expressed in liver hepatocytes on the basal-lateral side of the sinusoidal membrane. It serves to bring bile salts and other substrates from the blood stream into the liver.

Optivia offers NTCP substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for NTCP inhibitors as well as Vmax and Km values for NTCP substrates.

NTCP Transporter Assay

Relevance – Hepatic bile salt uptake Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Taurocholate
Subcellular Localization in Assay Model – Basolateral Inhibition Positive Control – NaCDC