NET Transporter Assay (SLC6A2)

Norepinephrine (NE) is synthesized from dopamine by dopamine beta-hydroxylase and stored into vesicles. Under normal circumstances, neuronal activation promotes the vesicular release of norepinephrine (NE) into the synapse. The norepinephrine transporter (NET; SlC6A2) is responsible for re-uptake of NE into the presynaptic neuron, and terminates its action. NET transporter is found in central and peripheral neurons, adrenal gland and placenta.

Optivia offers NET substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for NET inhibitors as well as Km and Vmax values for NET substrates.

NET Transporter Assay

Relevance – Neurotransmission; validated therapeutic target Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Dopamine
Subcellular Localization in Assay Model – Basolateral Inhibition Positive Control – Desipramine