MATE2-K Transporter Assay (SLC47A2)

The MATE2-K transporter is the multi-drug and toxin extrusion transporter that is encoded by the SLC47A2 gene. MATE2-K is expressed in the apical membrane in the kidney and can mediate elimination of substrates.

Optivia offers MATE2-K substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for MATE2-K inhibitors as well as Vmax and Km values for MATE2-K substrates.

MATE2-K Transporter Assay

Relevance – Renal tubular secretion Single or Multi-Transporter Model – Single
Transporter Type – Efflux Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Metformin
Subcellular Localization in Assay Model – Apical Inhibition Positive Control – Cimetidine