MATE2-K Transporter Assay (SLC47A2)
The MATE2-K transporter is the multi-drug and toxin extrusion transporter that is encoded by the SLC47A2 gene. MATE2-K is expressed in the apical membrane in the kidney and can mediate elimination of substrates.
Optivia offers MATE2-K substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for MATE2-K inhibitors as well as Vmax and Km values for MATE2-K substrates.
MATE2-K Transporter Assay
| Relevance – Renal tubular secretion | Single or Multi-Transporter Model – Single |
| Transporter Type – Efflux | Cell Model – MDCK-II |
| Assay Type – Intracellular accumulation | Exactly Matched Control Cells – Yes |
| Species – Human | Probe Substrate – Metformin |
| Subcellular Localization in Assay Model – Apical | Inhibition Positive Control – Cimetidine |