ENT2 Transporter Assay (SLC29A2)

The ENT2 transporter is a nucleoside equilibrative transporter that is encoded by the SLC29A2 gene. ENT2 is expressed widely throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides. ENT2 is also localized in the blood brain barrier and can be responsible for hydrophilic compounds entering the brain.

Optivia offers ENT2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for ENT2 inhibitors as well as Vmax and Km values for ENT2 substrates.

 ENT2 Transporter Assay

Relevance – Nucleoside transport Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human, rat Probe Substrate – Hypoxanthine
Subcellular Localization in Assay Model – Basolateral Inhibition Positive Control – Adenosine