ENT1 Transporter Assay (SLC29A1)

The ENT1 transporter is a nucleoside equilibrative transporter that is encoded by the SLC29A1 gene.  ENT1 is widely expressed throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides.

Optivia offers ENT1 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for ENT1 inhibitors as well as Vmax and Km  values for ENT1 substrates.

ENT1 Transporter Assay

Relevance – Nucleoside transport Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – Yes
Species – Human Probe Substrate – Adenosine
Subcellular Localization in Assay Model – Apical (and Basolateral) Inhibition Positive Control – NBMPR