CNT3 Transporter Assay (SLC28A3)
The CNT3 transporter is a nucleoside concentrative transporter that is encoded by the SLC28A3 gene. CNT3 is expressed widely throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides. CNT3 is also localized in the blood brain barrier and can be responsible for hydrophilic compounds entering the brain.
Optivia offers CNT3 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for CNT3 inhibitors as well as Vmax and Km for CNT3 substrates.
CNT3 Transporter Assay
|Relevance – Nucleosides salvage, nucleoside analog drugs intracellular accumulation||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake||Cell Model – MDCK-II|
|Assay Type – Intracellular accumulation||Exactly Matched Control Cells – Yes|
|Species – Human, rat||Probe Substrate – Uridine|
|Subcellular Localization in Assay Model – Apical||Inhibition Positive Control – Adenosine|
Clinical & Regulatory Relevance
CNT3 transporter is responsible for nucleoside salvage. CNT3 transporter plays a critical role in the cellular delivery of nucleoside analogs, including many antiviral and anticancer drugs such as gemcitabine and azacitidine.
Applications & Therapeutic Areas
Delivery of antiviral/anticancer drugs