CNT2 Transporter Assay (SLC28A2)
The CNT2 transporter is a nucleoside concentrative transporter that is encoded by the SLC28A2 gene. CNT2 is expressed widely throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides. CNT2 is also localized in the blood brain barrier and can be responsible for hydrophilic compounds entering the brain.
Optivia offers CNT2 substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for CNT2 inhibitors and Vmax and Km values for CNT2 substrates.
CNT2 Trasnporter Assay
|Relevance – nucleosides salvage, nucleoside analog drugs intracellular accumulation||Single or Multi-Transporter Model – Single|
|Transporter Type – Uptake||Cell Model – MDCK-II|
|Assay Type – Intracellular accumulation||Exactly Matched Control Cells – Yes|
|Species – Human, rat||Probe Substrate – Uridine|
|Subcellular Localization in Assay Model – Apical||Inhibition Positive Control – Adenosine|
Clinical & Regulatory Relevance
CNT2 transporter is responsible for nucleoside salvage. CNT2 transporter plays a critical role in the cellular delivery of nucleoside analogs, including many antiviral and anticancer drugs such as ribavirin and cladribine.
Applications & Therapeutic Areas
Delivery of antiviral/anticancer drugs