ASBT Transporter Assay (SLC10A2)

The ASBT transporter, apical sodium-dependent bile-acid transporter, is a member of the sodium/bile-salt co-transport family coded by gene SLC10A2. The transporter is electrogenic, coupled with sodium in a 2:1 sodium:bile acid stoichiometry. Human ASBT presumably functions as a monomer, although some evidence suggests the existence of a dimer as well.  ASBT is currently thought to be the primary mechanism for bile acid reabsorption in the intestine. ASBT is expressed at high levels in the terminal ileum, renal proximal tubules, and biliary epithelium.

Optivia offers ASBT substrate and inhibition assays for transport in polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for SERT inhibitors aw well as Vmax and Km for ASBT substrates.

ASBT Transporter Assay Details

Relevance – Bile acid uptake Single or Multi-Transporter Model – Single
Transporter Type – Uptake Cell Model – MDCK-II
Assay Type – Intracellular accumulation Exactly Matched Control Cells – yes
Species – Human Probe Substrate – Taurocholate
Subcellular Localization in Assay Model – Apical Inhibition Positive Control – NaCDC