Proven, high-quality ABC transporter vesicles manufactured by GenoMembrane
Quality assured — functionality of every batch verified by Optivia
Unmatched technical support from Optivia
Optivia is partnering with GenoMembrane Co., Ltd. (Japan) to offer their proven, high-quality transporter vesicle products in the US and Canada. These "inside-out" vesicles are ideal for studying efflux transporters, and can be used to complement Optivia's TransFlex™ cell-based transporter products.
Unlike other distributors, Optivia provides further assurance of product quality by performing rigorous functional testing on each batch of products shipped from Japan. Furthermore, Optivia offers competitive pricing, unmatched technical support, and attentive customer service to ensure that you get the most out of your vesicle assays.
Transporter Species Optivia Cat. # BCRP (ABCG2) Human OPT‐VGM0008 Rat OPT‐VGM0007 BSEP (ABCB11) Human OPT‐VGM0005 Rat OPT‐VGM0006 Dog OPT‐VGM0019 MDR1/P-gp (ABCB1) Human OPT‐VGM0015 Mouse—Mdr1a OPT‐VGM0004 Mouse—Mdr1b OPT‐VGM0016 MRP1 (ABCC1) Human OPT‐VGM0010 Rat OPT‐VGM0011 Dog OPT‐VGM0017 MRP2 (ABCC2) Human OPT‐VGM0001 Rat OPT‐VGM0002 Dog OPT‐VGM0014 Mouse OPT‐VGM0022 Monkey OPT‐VGM0018 MRP3 (ABCC3) Human OPT‐VGM0021 MRP4 (ABCC4) Human OPT‐VGM0012 Rat OPT‐VGM0020 MRP8 (ABCC8) Human OPT‐VGM0013 Control — OPT‐VGM0003
Why ABC Transporter Vesicles?
- Easily assess your compound’s interactions with efflux transporters
- Convenient, cell-free system
- High signal-to-noise ratios
- Satisfy EMA guideline recommendations for secondary (non-cell-based) systems
ATP-Binding Cassette (ABC) Transporter Vesicles allow you to easily evaluate your compound’s interactions with a given efflux transporter. Expertly produced by GenoMembrane, these inverted/inside-out vesicles are prepared from Sf9 insect cells engineered to overexpress individual efflux transporters. In cell-based systems, efflux proteins typically transport compounds out of the cells and into the environment. In an inverted vesicle format, however, the proteins are re-oriented along with the inverted membranes. As a result, they bind to the substrate in the solution surrounding the vesicles, and transport it into the vesicles where it accumulates. Quantitation of the accumulated compound allows you to determine whether your compound acts as a substrate and/or inhibitor of the transporter in question. The high levels of transporter activity and low background activity associated with these vesicles typically result in high signal-to-noise ratios, providing clear, unambiguous results. Please note, however, that highly permeant compounds may diffuse into or out of the vesicles, independent of transporter activity, minimizing differences between transporter expressing and control vesicles, potentially resulting in false negative results.
In addition to being a convenient, cell-free system with which to evaluate your compound, vesicular systems provide a secondary system that can be used to satisfy the EMA’s recommendation for the assessment of P-gp inhibition, and possibly that of other transporters. According to EMA guidelines1, because of the high inter-laboratory variability in the estimation of P-gp inhibition, “the use of two separate systems is recommended for this transporter…. [and] could also be considered for other transporters if indicated.”